Western Blot of iNOS in whole cell lysate (10 µg) from DLD-1 and DLD-1 induced (IFN-gamma (56 ng/mL), IL-1beta (145 pg/mL), and TNFa (10 ng/mL)). Samples were treated with NETN lysis buffer, incubated with iNOS monoclonal antibody (Product # MA5-44544) at a dilution of 1:1000, followed by HRP-conjugated goat anti-rabbit IgG (exposure time of 30 seconds) Lower Panel: Rabbit anti-COPB2.